Hs 578Bst 細(xì)胞，人正常乳腺細(xì)胞

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Hs 578Bst 細(xì)胞，人正常乳腺細(xì)胞

Organism	Homo sapiens, human
Tissue	mammary gland/breast
Cell Type	Epithelial, Myoepithelial
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1
Disease	normal
Age	74 years
Gender	female
Ethnicity	Caucasian Hs 578Bst 細(xì)胞，人正常乳腺細(xì)胞
Storage Conditions	liquid nitrogen vapor phase

Karyotype	modal number = 46; range = 42 to 48 This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%. Both X chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.

Derivation	Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126). Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo.
Receptor Expression	Epidermal growth factor (EGF)
Comments	No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected. A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximay 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.

Complete Growth Medium	The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subction Ratio: A subction ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week HTB-125 Hs 578Bst 人正常乳腺細(xì)胞 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. HTB-125 Hs 578Bst 人正常乳腺細(xì)胞
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%

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