AV无码久久久久不卡网站毛片,无码精品人妻一区二区三区漫画,久久精品国产99国产精品,全免费A级毛片免费看无码

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HUV-EC-C人臍靜脈內(nèi)皮細(xì)胞

人臍靜脈內(nèi)皮細(xì)胞

簡(jiǎn)要描述:CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):HUV-EC-C
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-26
  • 訪  問(wèn)  量:2975

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number: CRL-1730™ Price: $280.00

Designations: HUV-EC-C

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

Source: Organ: umbilical vein

Tissue: vascular endothelium

Disease: normal

Cell Type: endothelial

Cellular Products: factor VIII [23284]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (technology from amaxa)

Tumorigenic: No

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16

Cytogenetic Analysis: This is a hypodiploid human cell line. The modal chromosome number

was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments: Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Two to three times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682

29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915

33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443



CRL-1730 HUV-EC-C


























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
热RE99久久精品国产99热| 乱中年女人伦AV三区| 久久久久久久精品无码AV少妇| 国产欧美日韩中文久久| 亚洲熟女综合一区二区三区| 欲妇荡岳丰满交换| 97人人模人人爽人人喊免费官方| 色婷婷7777免费视频在线观看| 97精品国产自产在线观看永久| 亂倫近親相姦中文字幕| 久久伊人蜜桃AV一区二区| JK萌白酱在椅子上喷水视频| 无码熟妇人妻AV在线影片软件| 解开人妻的裙子猛烈进入| 黑人巨茎大战欧美白妇| 中文字幕精品久久久乱码乱码| 东京热人妻系列无码专区| 99久久蜜桃AV毛片毛片| 狠狠精品久久久无码中文字幕| 成人无码网WWW在线观看软件| 亚洲暴爽AV天天爽日日碰| 国产精品久久综合亚洲AV| 啊灬用力灬啊灬啊灬啊灬A片男男| 无码人妻精品一区二区三区66| FREE性朝鲜娇小VIDEOS| 免费A级毛片黄A片高清在线播放| 18款大禁用软件APP破解版| 少妇又色又紧又爽又刺激视频| 丰满人妻一区二区三区免费视频| 88久久精品无码一区二区毛片| 亚洲乱码国产乱码精品精大量| 同桌上课脱裙子让我帮他自慰| 久久久噜噜噜久久熟女AA片| 波多野结衣视频| 破外女13一14在线观看| 欧美人与动XXXXZ0OZ| 无码人妻AV免费一区二区三区| 青春草无码精品视频在线观看| YELLOW日本高清在线| 久久久久久曰本AV免费免费| 国产精品99久久久久久猫咪|